Axen AH, Pierce NE.
Aggregation as a cost-reducing strategy for lycaenid larvae. Behavioral Ecology. 1998;9 :109-115.
AbstractIf a mutualistic relationship entails providing services at a cost, selection will favor individuals that maximize the net benefits of the interaction and minimize the costs. Larvae of many species of lycaenid butterflies secrete nutritious food rewards to attending ants and, in return, receive protection against predators and parasitoids. Because ants typically recruit more workers to larger resources, by forming groups the larvae may ensure more reliable access to ants and thereby gain better protection. A further consequence of aggregating should be a change of the cost-benefit relationship for individual larvae. The larger the group, the smaller a single larva's influence will be on total ant density, which could lead to a smaller investment in secretion, thus reducing the per capita cost of cooperation. In this study, the influence of ant attendance, group size, and companion quality on larval investment was investigated. The interaction between the obligately ant-dependent lycaenid, Jalmenus evagoras, and its attendant Iridomyrmex ants was manipulated and the effect on larval secretion measured. As the level of ant attendance increased, the delivery of food rewards increased, both for solitary and for aggregated larvae. When aggregated, larvae provided less food rewards to ants than when solitary, and secretion rate decreased with increasing group size. Furthermore, larvae had lower secretion rates when paired with a bigger, more attractive larva than when paired with a smaller one. The considerable reduction in secretion rates for larvae in groups suggests that gaining protection at a lower secretion cost could be one factor that promotes aggregation in myrmecophilous lycaenids.
1998_axen_pierce.pdf Yu DW, Pierce NE.
A castration parasite of an ant-plant mutualism. Proceedings of the Royal Society B-Biological Sciences. 1998;265 :375-382.
AbstractExploring the factors governing the maintenance and breakdown of cooperation between mutualists is an intriguing and enduring problem for evolutionary ecology, and symbioses between ants and plants can provide useful experimental models for such studies. Hundreds of tropical plant species have evolved structures to house and feed ants, and these ant-plant symbioses have long been considered classic examples of mutualism. Here, we report that the primary ant symbiont, Allomerus cf. demerarae, of the most abundant ant-plant found in south-east Peru, Cordia nodosa Lam., castrates its host plant. Allomerus workers protect new leaves and their associated domatia from herbivory, but destroy flowers, reducing fruit production to zero in most host plants. Castrated plants occupied by Allomerus provide more domatia for their associated ants than plants occupied by three species of Azteca ants that do not castrate their hosts. Allomerus colonies in larger plants have higher fecundity. As a consequence, Allomerus appears to benefit from its castration behaviour, to the detriment of C. nodosa. The C. nodosa-ant system exhibits none of the retaliatory or filtering mechanisms shown to stabilize cheating in other cooperative systems, and appears to persist because some of the plants, albeit a small;minority, are inhabited by the three species of truly mutualistic Azteca ants.
1998_yu_and_pierce.pdf Townson SM, Chang BSW, Salcedo E, Chadwell LV, Pierce NE, Britt SG.
Honeybee blue-and ultraviolet-sensitive opsins: Cloning, heterologous expression in Drosophila, and physiological characterization. Journal of Neuroscience. 1998;18 :2412-2422.
AbstractThe honeybee (Apis mellifera) visual system contains three classes of retinal photoreceptor cells that are maximally sensitive to light at 440 nm (blue), 350 nm (ultraviolet), and 540 nm (green), We performed a PCR-based screen to identify the genes encoding the Apis blue- and ultraviolet (UV)-sensitive opsins, We obtained cDNAs that encode proteins having a high degree of sequence and structural similarity to other invertebrate and vertebrate visual pigments. The Apis blue opsin cDNA encodes a protein of 377 amino acids that is most closely related to other invertebrate visual pigments that are thought to be blue-sensitive. The UV opsin cDNA encodes a protein of 371 amino acids that is most closely related to the UV-sensitive Drosophila Rh3 and Rh4 opsins. To test whether these novel Apis opsin genes encode functional visual pigments and to determine their spectral properties, we expressed them in the R1-6 photoreceptor cells of blind ninaE mutant Drosophila, which lack the major opsin of the fly compound eye. We found that the expression of either the Apis blue- or UV-sensitive opsin in transgenic flies rescued the visual defect of ninaE mutants, indicating that both genes encode functional visual pigments. Spectral sensitivity measurements of these flies demonstrated that the blue and UV visual pigments are maximally sensitive to light at 439 and 353 nm, respectively. These maxima are in excellent agreement with those determined previously by single-cell recordings from Apis photoreceptor cells and provide definitive evidence that the genes described here encode visual pigments having blue and UV sensitivity.
townson_opsins.pdf